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FC latency & bucket access today (July 18 2018)

Hi FC team, We have noticed a dramatic slowdown of FC workspace/method config loading, and intermittent but frequent "bucket unavailable" messages popping up in various places, including blocking us...

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Errors related to connection issues internet proxy

I have aggregate_bismark_output_grch38 method configuration in aryee-merkin/dna-methylation-pipeline-paper workspace. It uses some bioconductor packages to access annotation information from their...

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Is queueing broken?

I started a set of preemptible scatter jobs yesterday, but these failed in large numbers with the error "The job was stopped before the command finished. PAPI error code 3. Message: You cannot create...

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Getting lots of "PAPI error code 10. Message: 14" errors

Many of the jobs I launched last night have failed with "PAPI error code 10. Message: 14" errors. First, can we change this error message to something more helpful? And do we know what this error means...

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Unable to create a new FireCloud workspace

I've created a new billing account, epilepsy-sc-neale, attached to CO 6910090. I go through the steps to add a new workspace, named Epilepsy_​Busch_ClevelandClinic_GSA-MD_QC, and I'm getting an error...

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Slowdown & 15m periodicity when checking call cache

Hi FC Team, In the last ~6 hours, I've noticed some unusual behavior when rerunning workflows almost entirely from cached results. This new behavior is causing these workflows to go from rerunning...

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Error! You do not have access to this file.

Hi Yesterday I updated 55 WGS bams in a bucket. I copied the files from /seq/picard_aggregation and verified that the files are in the bucket using gsutil from my laptop, (also from Broad servers, as...

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Ownership of Entities in FireCloud

Hi, In transitioning users from Firehose to FireCloud we have been asked about the following: Who owns the data and methods in the FireCloud system? Does the Broad claim any ownership of the data or...

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Slow Call Cache Checking

I've been noticing very slow call cache checks this afternoon. I submitting about 80 samples to a workflow that should have a fair number of call cache hits and then noticed that a subset of these...

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request to whitelist Firecloud workspace for high core/high mem GC

We're assessing the performance of 10x genomics software (CellRanger) to process single cell RNAseq data. Using a 64core 240G VM, it takes 7 hours to run our pipeline (down from 12 hours for 32 core,...

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Need to include custom interval list in...

Not sure if this is a GATK question or Firecloud question, but: According to this article, it is recommended that a set of intervals be used with BQSR when working with exomes. However, in the best...

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how to handle multiple ubam files for a sample, each represent a read group.

Please see if following data model files are properly formated participate file: entity:participant_id disease gender age MORL-X6031_50 Otoscope M 53 sample file: entity:sample_id participant_id...

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"rawls" error

Hello, When I try to run a method configuration, the following error message appears: cannot find credentials for RawlsUser(RawlsUserSubjectId(105492220035590692689),RawlsUserEmail(******)) I believe I...

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PAPI error code 5. Message: 10: Failed to delocalize files: failed to copy...

I keep receiving this error when attempting to run my WDL script. I have run the WDL locally without error using the same docker image. I have permission to the files in the google bucket. I believe it...

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unable to reference a bucket object directly in method config

I have a method config that references a file input through a workspace attribute. I wanted to replace the attribute value with an explicit google bucket URL. I got the following error when I saved the...

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How to create sample file for samples with multiple ubams, each represent a...

Hi I have WGS samples, each have multiple ubams and each of them for one read groups. My question is how to create sample file for metadata?

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Breaking change in Cromwell34 - can the version be chosen?

Today's surprise update to Cromwell34 not only killed all of our existing workflows, but appears to have introduced a breaking change: Expecting memory runtime attribute to be an Integer or String with...

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Getting the same New Data email

I've gotten 7 emails in the past hour of the same message Dear FireCloud user, Heads up! New data has been added to Cellopedia_CIRM_Eggan_iPSC_WGS. Thanks for using FireCloud!

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Call caching not working reliably.

I ran a multi-task workflow on a sample pair yesterday. The workflow failed due to the Cromwell 34 upgrade. Several of the tasks in that workflow, however, had succeeded prior to the upgrade-induced...

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Problem in running five-dollar-genome-analysis-pipeline

The running failed: X6031_50_2_8 Workflow ID:2afe3bf7-9d41-4ae9-b934-11b064bebeef Status: Failed Total Run Cost:n/a Submitted:July 31, 2018, 2:20 PM (1 hour ago) Started:July 31, 2018, 3:39 PM (36...

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